Life Sciences & Biotechnology
Title : | Role of RUN and FYVE domain-containing protein RUFY1 in cargo sorting and transport to Golgi and Golgi-derived storage vesicles |
Area of research : | Life Sciences & Biotechnology |
Principal Investigator : | Dr. Mahak Sharma, Indian Institute Of Science Education And Research (IISER) Mohali, Punjab |
Timeline Start Year : | 2022 |
Timeline End Year : | 2025 |
Contact info : | msharma@iisermohali.ac.in |
Details
Executive Summary : | Cargo trafficking is a complex process that involves sorting, packing, budding, motility, tethering, and fusion with the target compartment. Small GTP-binding proteins, such as Rabs, Arfs, and Arf-like (Arl) GTPases, play a crucial role in this process by recruiting effector proteins to specific organelles/endosomes. One effector protein, RUFY1, is involved in cargo sorting at the early endosome. RUFY1 belongs to the RUFY family of proteins, with four members in mammals. No studies have explored whether RUFY1 regulates cargo sorting from endosomes to the target compartment (TNG). However, RUFY1 has been established to regulate CI-M6PR trafficking from endosomes to the TGN, and depletion of RUFY1 leads to enlarged lysosomes. RUFY1 overexpression in adipocytes promotes insulin-stimulated surface translocation of glucose transporter GLUT4, which mediates glucose uptake in muscle, adipose, and cardiac tissues following insulin-stimulated translocation from intracellular sequestration pool known as GLUT4 storage vesicles (GSVs). The study aims to study the role of RUFY1 in the trafficking of Glucose transporter 4 (GLUT4), which plays a crucial role in glucose homeostasis. Endosomal sorting and retrograde transport to TGN are also involved in GLUT4 trafficking and the formation of GSVs. The study also aims to characterize whether RUFY1 is a dynein-dynactin motor adaptor and whether its role in mediating dynein-based motility is essential for retrograde cargo transport from endosomes to the TGN. |
Total Budget (INR): | 59,01,808 |
Organizations involved