Life Sciences & Biotechnology
Title : | Prospective Profiling of Circulating Dendritic Cell Subsets for Early Detection of Early onset and Late onset Pre-eclampsia Among Pregnant Women |
Area of research : | Life Sciences & Biotechnology |
Principal Investigator : | Dr. Aarthi Sundararajan, Nirma University, Ahmedabad, Gujarat |
Timeline Start Year : | 2022 |
Timeline End Year : | 2024 |
Contact info : | aarthisundararajan@gmail.com |
Equipments : | Liquid Nitrogen Container
Laptop |
Details
Executive Summary : | Pre-eclampsia (PE) is a pro-inflammatory immune disorder that causes maternal-fetal mortality and morbidity. It is classified into two subtypes based on the time of onset of clinical symptoms: early onset (EOPE) before 34th gestation week and late onset (LOPE) at or after 34th gestation week. Studies show differential pathogenesis, clinical symptoms, and immune profile between EOPE and LOPE patients. There is a need to identify novel biomarkers to diagnose EOPE and LOPE among pregnant women. This will enable designing immunotherapeutic treatment strategies for these two subtypes. Human dendritic cell (DC) subsets imbalance mediates pro-inflammatory disorders and induces hypertension among cardiovascular disease patients. The role of DC subsets in PE, particularly EOPE vs LOPE pathogenesis, is unclear. Preliminary studies indicate an imbalance of circulating DC subsets during the third trimester among clinically diagnosed EOPE pregnant women compared to gestation age matched normal pregnant women. However, questions remain about how early during pregnancy DC subset imbalance could be detected among women with EOPE outcome and how these subsets differ in women with LOPE outcome who exhibit delayed onset of hypertension and pro-inflammation. This proposal evaluates these questions through a prospective, longitudinal study design in a hospital setting. Eligible pregnant women (n=400) will be enrolled with informed consent, and blood samples will be collected at two time points during pregnancy. Post-partum information will be recorded, and the profile of DC subsets and clinical parameters collected will be compared between women with EOPE, LOPE, and normal birth outcomes. |
Total Budget (INR): | 31,59,170 |
Organizations involved