Life Sciences & Biotechnology
Title : | Profiling and Development of Exosome-based Novel Anti-arthritic Flavonoid Formulation to Mitigate the Hif Regulated Angiogenic-pannus Growth in Rheumatoid Arthritis |
Area of research : | Life Sciences & Biotechnology |
Principal Investigator : | Dr. Dinesh Kumar L, Bannari Amman Institute Of Technology, Tamil Nadu |
Timeline Start Year : | 2022 |
Timeline End Year : | 2024 |
Contact info : | dineshbt28@gmail.com |
Equipments : | Cooling centrifuge
UV-Vis Spectrophotometer
Micropipettes
pH meter
Weighing balance |
Details
Executive Summary : | Rheumatoid arthritis (RA) is an autoimmune disorder characterized by degenerative musculoskeletal joint disease, leading to serious comorbidities in vital organs like the lungs and heart. Current treatments for RA include biological Disease Modifying Anti-Rheumatic Drugs (bDMARDs) and new targeted therapeutics in the late-stage pipeline. However, these treatments often fail to reduce inflammation and may relapse after withdrawal. There is a need for more efficient drugs targeting regulatory targets such as High-Mobility Group Box 1-Hypoxia-Inducible Factor (HMGB1/HIF-1α) and GATA4 transcription factor, which influence VEGF and other angiogenic mediators to promote or restrain vascularization, edema, and pannus formation. The HMGB1/HIF-1α pathway is one of the main progressive pathways of RA, triggering critical events like synovial hyperplasia and bone destruction. Flavonoids, polyphenolic plant compounds known for their anti-inflammatory and immunomodulatory effects in autoimmune diseases, are being studied for their disease suppressing effects in RA. Bioactive flavonoids will be collected from standard research literature and analyzed for their pharmacokinetic properties. The association of RA-angiogenesis (RA-Ag) regulatory targets with other pathway mediators and bioactive small molecules will be assessed using network pharmacology. The possible mechanism of the flavonoids and their predicted RA-Ag targets will be assessed through interaction and stability analysis by molecular docking and dynamics studies. The modulatory efficiency of the compound in the RA-Ag pathway will be studied using in vitro cell culture. Effective flavonoid compounds will be formulated as drugs or supplements to withhold the progression and severity of RA. |
Total Budget (INR): | 20,90,000 |
Patents : | 1 |
Organizations involved