Research
Title : | Modelling of indigenous diagnostics and immuno-potent vaccine candidates to combat African swine fever in India |
Area of Research : | Medical Sciences |
Focus Area : | Veterinary Biotechnology, Vaccine Development |
Principal Investigator : | Dr. Lukumoni Buragohain, Assam Agricultural University (AAU)Khanapara, Guwahati, Assam; Dr. Nagendra Barman, College of Veterinary Science, Khanapara, Guwahati, Assam; Dr. Sachin Kumar, Indian Institute of Technology (IIT), Guwahati, Assam; Dr. Yashpal Malik, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab |
Co-PI: | Dr. Durlav Bora, College of Veterinary Science, AAU, Khanapara, Guwahati, Assam; Dr. Biswajit Dutta, College of Veterinary Science, AAU, Khanapara, Guwahati, Assam; Dr. Shirisha Nagotu, Indian Institute of Technology (IIT), Guwahati, Assam; Dr. Niraj Singh, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana; Dr. Ratan Choudhary, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana; Dr. BV Kumar, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana; Dr. Probodh Borah, College of Veterinary Science, AAU, Khanapara, Guwahati, Assam; Dr. Sophia Gogoi, College of Veterinary Science, AAU, Khanapara, Guwahati, Assam; Dr. Rupam Dutta, College of |
Contact info : | lukumoni54@gmail.com; sachin22vet@gmail.com; sachinku@iitg.ac.in; malikyps@gmail.com; nnbarman@gmail.com; drdpbora@gmail.com; drbiswajitkvk@gmail.com; snagotu@iitg.ac.in,nirajvet57@gmail.com; vetdrrkc@gmail.com; drbvskumar@gmail.com; borahp@vetbifg.ac.in; sophiagogoi@gmail.com; drrupamdutta@gmail.com |
Timeline Start Year : | 2021 |
Timeline End Year : | 2024 |
Total Budget (INR): | 21,5.,16,721 |
Details
Executive Summary : | African swine fever (ASF) is a contagious viral disease of pigs, which can cause mortality approaching 100%. ASF is a serious threat to the global pig industry and its frequent outbreaks in the globe is imparting serious economic impact on pig rearing farmers. Recently, the outbreaks of ASF in Assam and Arunachal Pradesh have nudged the piggery sector of northeast India. One of the best possible ways to control outbreak of infectious diseases is early and rapid diagnosis as well as vaccination of healthy population. As of now, there is no indigenous diagnostic assay or vaccine for ASF available in India, as ASF occurred for the first time in our country. Keeping in view these facts, this project is proposed to develop indigenous protein and nucleic acid based diagnostic assays for real-time detection of ASFV at laboratory and field condition as well as to identify and characterize certain ASFV antigens having potential to be used as vaccine candidate. |
Equipments : | Luminex System, Cell analyzer, Ultra-centrifuge |
Achievements : | Cloning, expression, purification, and characterization of immunogenic proteins p30 andp54 of African Swine Fever Virus (ASFV) in abacterial expression system has been done. Construction, recovery, and characterization of recombinant NDV strain B1/lentogenic NDV cDNAs and recombinant adenovirus expressing immunogenic structural proteins of ASFV have been developed. The potential NDV vectored vaccine candidates exhibited promising humoral and cell-mediate immune response in pigs. Molecular characterization of ASFV circulating in 8 states of NER has been done and it was observed that in the states of NER the genotype-II is in circulation. The vaccine candidates both protein cocktail and NDV vectored exhibited promising and broad-spectrum immune response. The indirect ELISA optimized to detec |
Outcome/Output: | 1 |
Organizations involved
Implementing Agency : | College of Veterinary Science, Assam Agricultural University, Guwahati, Assam Funding Agency :Department of Science and Technology (DBT) | Source: E-promis and Information received by Investigator | |