Research
Title : | Incorporation of crtRB1 allele into sweet corn inbreeds and north eastern land races for development of bio-fortified sweet corn (E28AHE) |
Area of Research : | Agricultural Sciences |
Focus Area : | Biofortification, Plant Biotechnology |
Principal Investigator : | Dr. Pramesh Khoyumthem, College of Agriculture, Central Agricultural University (CAU), Imphal, Manipur; Dr. Senthil Natesan, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu |
Co-PI: | Dr. Ravikesavan Rajasekaran, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu; Dr. Vellaikumar Sampathrajan, Agricultural College and Research Institute, Madurai, Tamil Nadu |
Contact info : | prameshkh@gmail.com; senthil_natesan@yahoo.com; chithuragul@gmail.com; vellai1973@yahoo.co.in |
Timeline Start Year : | 2022 |
Timeline End Year : | 2025 |
Total Budget (INR): | 54,.2,836 |
Details
Executive Summary : | Among the specility corns, the sweet corn gaining importance due to changing food habits and preparation methods in the urban population.The demand for sweet corn is quite high in city hotels and also found shelf space in big malls in the city. Hence the sweet corn is a better target for the biofortification program.The NEH maize Yaribok Chujak having high beta carotene content (Senthil et al. 2021 unpublished) and Chujak makkai (violet colour) will be used for the addition of the sh2 trait to incorporate sweetness. Beta carotene and sugar estimation will be done at 75DAS. The NEH landraces having various anthocyanin color grains will be used for the addition of sh2 alleles for sweetness which will open up new market potential. The sweet corn inbred USC1-2-3-1 and SC 12039-1 at TNAU will be introgressed with beta carotene trait using the MABB. The crtRB1 and sh2 gene-specific marker will be used for the selection of homozygous lines for beta carotene trait and sweetness respectively. |
Equipments : | NA |
Achievements : | The present work focused on the development of nutritionally enriched sweet corn through introgression of β-carotene, sweetness (sh2), and anthocyanin traits into elite and locally adapted genetic backgrounds. A set of six β-carotene donor lines (DBT15, DBT16, DBT17, DBT18, DBT23,DBT25) were utilized to develop the hybrid CSBH 23004, which recorded a 10% yield increase over the commercial check Misthi in PYT–Kharif 2023 and CYT–Summer 2024. Owing to its superior performance, CSBH 23004 was nominated for MLT–Kharif 2024. Parallel efforts targeted the NEHR landrace Yaripok chujak (CAUM66), where advanced backcross lines of CAUM66β+ × DBT16carrying sh2 and crtRB1 (CAUM66/DBT16-5-6-8-3-112-2,-11-2-26-13-110-7,-24-58-3-17-111-15,-23-59-6-34-116-9) were developed. These lines expressed 14–16 μg/ |
Outcome/Output: | 2 |
Organizations involved
Implementing Agency : | Central Agricultural University, Imphal, Manipur Funding Agency :Department of Science and Technology (DBT) | Source: E-promis and Information received by Investigator | |