Research
Title : | Development of Disease Diagnostic Kits for CTV, HLB, Phytophthora Gummosis and Citrus canker in Citrus of North East India |
Area of Research : | Agricultural Sciences |
Focus Area : | Plant Disease Diagnostics |
Principal Investigator : | "Prof. Lingaraj Sahoo, Indian Institute of Technology (IIT), Guwahati, Assam; Dr. Rahul Hepat, Institute of Advanced Study in Science and Technology (IASST), Guwahati, Assam; Dr. Dwipendra Thakuria, CPGSAS, Central Agricultural University(Imphal), Umiam, Meghalaya; Dr. Lalit Laxman Kharbikar, ICAR- National Institute of Biotic Stress Management (NIBSM), Raipur, Chhattisgarh; Dr. Dinesh Singh, ICAR- Indian Agricultural Research Institute (IARI), New Delhi" |
Contact info : | ls@iitg.ernet.in; rhepat@gmail.com; thakuria.dwipendra@yahoo.co.in; lalitkharbikar@gmail.com; dinesh_iari@rediffmail.com |
Timeline Start Year : | 2021 |
Timeline End Year : | 2025 |
Total Budget (INR): | 2,82,.8,504 |
Details
Executive Summary : | The aims of the research proposal are: (a) develop a simple and cost-efficient diagnostic immuno-strip based diagnostic kit in dip-stick format for detection of CTV strains in infected plant parts both in nursery and orchard; (b) Identification of CTV strains from various hotspot regions of NER and development of artificial Agroinfectious dimeric clones for Agroinoculation in various Citrus germplasm for testing CTV susceptibility; (c) development of a LAMP-based Point-of-care diagnostic kit for detection of Phytophthora Gummosis, Canker and citrus greening (HLB) through systematic genetic diversity studies and (d) formulation of liquid synthetic microbial consortium’ for addressing these diseases. |
Equipments : | Real-Time PCR |
Achievements : | An extensive survey was undertaken during 2021-22 and 2022-23 across major citrus-growing regions of Northeast Indiax, Meghalaya, Tripura, Manipur, Arunachal Pradesh, Sikkim, Nagaland and Assam, along with Punjab, Haryana and Delhi to investigate the causal agent of citrus root rotand gummosis. A standardized isolation method for Phytophthora spp. was developed using young lemon-leaf baiting on corn meal agar supplemented with pimaricin(10μg/ml), ampicillin (250μg/ml), rifamycin (10μg/ml) and carbendazim(300μg/ml).A total of 101 isolates were successfully obtained, maintained on carrot agar and preserved in sterile water at 4 °C. Morphological characterization revealed fast-growing colonies with chrysanthemum and rosette patterns and whitish blooming mycelia. Pathogenicity assays on detach |
Outcome/Output: | 1 |
Organizations involved
Implementing Agency : | Indian Institute of Technology(IIT), Guwahati, Assam Funding Agency :Department of Science and Technology (DBT) | Source: E-promis and Information received by Investigator | |