IN372958: 6-substituted-7-hydroxy-4-(methylthio)-2-oxo-2h-chromene-3-carbonitriles useful as vacuoles staining dyes

The invention relates to new 6-substituted-7-Hydroxy-4-(methylthio)-2-oxo-2Hchromene-3-carbonitriles of the general formula I which can be used potentially as fluorescent probes, diagnostics, fluorescent tags, for staining of vacuoles of virus particles, bacterial particles, virus components, biological cells (such as animal cells, plant cells, bacteria, or yeast), or cellular components in fluorescence-based imaging and/or analysis in fixed or live cell. Wherein R is independently selected from the groups consisting of, fluoro, chloro, bromo, iodo, hydroxy, carbontrifluoride, C1-C8 alkyl.