Artificial bidirectional promoter for activation of gene expression

A bidirectional module for activation of gene expression and regulation of transcription in both directions is disclosed. The bidirectional module comprises multiple cis regulatory DNA sequence elements, strategically arranged to give a `Transcription Activating Module` that achieves high level expression from a `Transcription Initiation Module. The latter functions like a minimal promoter. The former activates transcription simultaneously in both the directions from the latter and also responds to several transcription inducing, external stimuli in both the directions. Since it is an artificially designed bidirectional transcription module, it has no equivalent DNA sequence in plant genome. This reduces the chances of the genes from being silenced by homology based mechanisms. A bidirectional promoter module as this, can therefore be used to develop efficient vectors for genetic engineering in plants.

Filing Date: 03-10-2006

Issue Date: 24-06-2008

Patent No: US7390661 [USA]

Application No: 11/542,590

Inventor(s): Tuli Rakesh; Vishwanath Sawant Samir; Chaturvedi Chandra Prakash; Kiran Kanti; Mehrotra Rajesh

Licensing Option: Laboratory willing to jointly develop technology with industrial partner.

Implementing institute: CSIR-National Botanical Research Institute [CSIR-NBRI]

Funding agency: Council of Scientific and Industrial Research (CSIR)

IPC Classification: C12N 5/14 (20060101), A01H 1/00 (20060101), C12N 15/00 (20060101), C12N 15/09 (20060101), C12P 21/06 (20060101), C12N 15/11 (20060101), C12N

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