Life Sciences & Biotechnology
Title : | Dissecting role of essential lysine acetyl transferase TIP60 as epigenetic modulator of tissue repair mechanism |
Area of research : | Life Sciences & Biotechnology |
Principal Investigator : | Dr. Ashish Gupta, Shiv Nadar Institution Of Eminence, Uttar Pradesh |
Timeline Start Year : | 2022 |
Timeline End Year : | 2025 |
Contact info : | ag315@snu.edu.in |
Details
Executive Summary : | The complex process of wound healing is characterized by a series of precisely regulated programmed events that partially overlap however proceed in proper sequence and time-frame. Within minutes of injury, cellular signals released by the immune cells mark the onset of the proliferation phase, which is characterized by re-epithelialization process that involves collective migration of epithelial cells over the wound site to restore the epithelial barrier eventually closing the wound. Although wound healing has been studied for decades and the stages of normal healing process are mostly known, we still lack clear understanding of regulatory networks and molecular players involved in this process so as to modulate them in a controlled manner for therapeutic purposes. It is necessary to characterize the course of these events at higher resolution in order to thoroughly understand the very complex aspects involved. Timing taken for wound re-epithelialization plays a crucial role in determining the outcome of the wound repair process and has been observed that faster re-epithelization leads to scarless repair. Our laboratory demonstrated an interesting finding that TIP60, an essential lysine acetyltransferase protein interacts and induces intranuclear reorganization of PXR, a class II nuclear receptor and together this complex enhanced cell migratory and adhesion properties (Bakshi K et al 2017). Recently we have identified that TIP60/PXR complex is required for rapid filopodia formation and enhanced cell migration during wound healing (unpublished data). Together, these preliminary finding forms the basis of our proposed research work, where we want to verify and elucidate the role and mechanism of TIP60/PXR directed wound repair process using in vivo mouse model system. Besides providing invaluable information on the molecular and cellular basis of these processes this will also help to interpret the results more accurately in natural setting using animal models. |
Total Budget (INR): | 18,30,000 |
Organizations involved