Life Sciences & Biotechnology
Title : | Mechanistic insight of healing diabetic wound by different varieties of honey via regulating the expression of adenosine monophosphate-activated protein kinase (AMPK), hypoxia inducible factor-1α (HIF-1α) and inflammatory cytokines with special emphasis on identification of active component in honey |
Area of research : | Life Sciences & Biotechnology |
Principal Investigator : | Dr. Bapi Gorain, Birla Institute Of Technology, Mesra, Jharkhand |
Timeline Start Year : | 2022 |
Timeline End Year : | 2024 |
Contact info : | bapi.gorain@bitmesra.ac.in |
Equipments : | "Inverted Microscope with 5 MP CMOS camera
Vertical -20 deep freezer
Hotplate Magnetic Stirrer" |
Details
Executive Summary : | Disruption of highly controlled and well-orchestrated process of wound healing resulted in the increasing risk of diabetic wounds, where manipulation in reactive oxygen species production, inflammatory condition, and vascularization of the wound environment delay its healing process. Investigation of natural components from ayurvedic scriptures to scientifically establish the underlying mechanism and to identify the active(s) is of utmost necessity in current therapy. Thus, the present investigation has been aimed to evaluate six different natural varieties of honey in healing diabetic wounds attempting on adenosine monophosphate-activated protein kinase (AMPK), hypoxia-inducible factor-1α (HIF-1α) and inflammatory pathways, where AMPK signaling pathway is known to promote vascular homeostasis, modulation of reactive oxygen species, protection against apoptosis, angiogenesis, diabetic wound healing; downregulation of HIF-1α expression in diabetic condition is known to cause poor healing of wounds; and delayed inflammatory phase results in impairment of matured granulation tissue formation and decreased in wound tensile strength. In addition, downregulation of HIF-1α decreases autophagy of AMPK activity independently, indicating AMPK and HIF-1α pathways work autonomously in the wound healing process. All these pathways present important molecular aspects of wound healing where the roles of different varieties of honey will provide the scientific evidence to identify the active(s) components of honey with diabetic wound healing potential. To achieve the set objectives, considering the physicochemical properties of honey, different varieties of honey will be incorporated into the biodegradable polymeric scaffold to facilitate in situ conversion of sol-to-gel by the stimuli at the wound environment which will eventually provide longer retention at the applied wound beds to release the entrapped honey at a sustained rate. A sustained supply of honey at the wound bed will modulate the healing mechanism, which is hypothesized to protect and heal the experimentally induced diabetic wound in animal model through AMPK, HIF-1α and inflammatory pathways. Western blotting of the wound biopsy samples would be performed using specific primary and secondary antibodies for the efficiency of expressing AMPK and HIF-1α. Alternatively, inflammatory cytokines (ILs and TNF-α) at the wound site will be estimated using ELISA techniques. These findings will be supported to establish the potent variety of honey in the healing of diabetic wounds, which will finally be analysed for its components to identify the active(s) following similar treatment in experimental wound model and molecular analysis of wound biopsies (AMPK, HIF-1α and inflammatory pathways). It is expected that the potent honey, identification of active component(s) and establishment of underlying mechanisms in diabetic wounds would meet the important concern from the social and clinical perspective. |
Total Budget (INR): | 29,40,335 |
Organizations involved