Life Sciences & Biotechnology
Title : | Isolation and characterization of densely veined leaf mutant(s) in rice (Oryza sativa L.) |
Area of research : | Life Sciences & Biotechnology |
Principal Investigator : | Dr. Selvakumar Gurunathan, SRM Institute Of Science And Technology, Tamil Nadu |
Timeline Start Year : | 2022 |
Timeline End Year : | 2025 |
Contact info : | selvakumaragri@gmail.com |
Equipments : | Microscope with Camera |
Details
Executive Summary : | Rice, the primary energy source for the human population, faces challenges due to climate change. The plant's C3 mode is inefficient, losing up to 25% of carbon during photorespiration. Temperatures exceeding 30°C can reduce photosynthetic efficiency by up to 40%, especially in rice. Elevated CO2 concentration limits stomatal conductance in rice. Leaf veins play a crucial role in physiological responses, and reduced interveinal density (IVD) is one of the earliest steps in the evolution of C4 photosynthesis. The rapid exchange of photosynthates in rice requires a good vascular network, optimal ratio of mesophyll and bundle sheath cells, and close contact. C4 plants have a reduced M:BS ratio, which facilitates rapid intercellular diffusion of metabolites. Modifying the C3 cycle and related traits is the best way to increase rice's photosynthetic ability. Vine density is positively correlated with higher photosynthesis values, so developing crops with sequential distribution of major and minor veins across leaf width is a good approach. Induced mutagenesis is the best way to generate novel mutations for vein density improvement. Mutagenesis in rice can provide an artificial source of novel genetic variation, allowing for the identification of rice plants with HVD.
This project aims to screen EMS-induced and stable rice mutants with ICAR-NIPB for the HVD trait, fulfilling the gap in efforts to generate ideal vein architecture in rice crops for improved photosynthetic efficiency. Combining MutMap analysis with transcriptome data will enable identification of mutations and genomic regions responsible for HVD in rice. |
Co-PI: | Dr. Mahendran R, SRM Institute Of Science And Technology, Tamil Nadu-603203; Dr. AmithaMithra V Sevanthi, ICAR-National Institute For Plant Biotechnology (NIPB), Delhi-110012 |
Total Budget (INR): | 33,88,880 |
Organizations involved