Executive Summary : | Exosomes are intracellular membrane-based vesicles with diverse compositions that are involved in biological and pathological processes.Exosomes is attractive nanocarriers for therapeutic usage due to their perfect natural structure and properties. Liposomes and polymeric nanoparticles are the most widely used drug delivery carriers for a variety of drugs. However, the ability of the liposomal and polymeric nanoparticle system to attack the host immune system, as well as its circulation and stability, without causing toxicity, is still unknown. Exosome-encapsulated with drug improved circulation time, therapeutic action, and transport to the brain. Exosomes could operate as an "invisibility cloak" for integrated therapeutic drugs, lowering MPS clearance while also improving drug distribution to target tissues. Exosomes may combine the advantages of synthetic nanocarriers and cell-mediated drug delivery systems, avoiding the toxicity and rapid clearance associated with synthetic vehicles, as well as the complexity of deploying cell-mediated drug delivery systems in the clinic. Mammalian exosomes have emerged as a promising class of functional materials, inspiring novel applications as therapeutic vehicles and nutraceutical compounds. Although this, their immunogenicity has been an issue of controversy within the scientific community. Though, exosome-like vesicles innately formed in plants and inherent to eukaryotic cell-derived vesicles, could soothe most of the concerns, they are notably underutilized as therapeutic modalities. In the present investigation, we would isolate Exosome Nanovesicles(EN) from Garlic(G), Colocasia esculenta(C) rhizome, Maize milk(M), and Potato(P) (S. tuberosum) by centrifugation and load them with Rotigotine using freeze thaw cycling and or ultrasonication before evaluating them for intranasal administration (nose to brain). Also, various physicochemical properties and distribution in the mouse brain, in vivo toxicity study, and therapeutic efficacy for Parkinson disease in mice would be investigated. Exosome Nanovesicles (EN) from Garlic (G), Colocasia esculenta rhizome(C), Maize milk (M), and Potato (P) (S. tuberosum) laden with Rotigotine would thus be a superior choice Nanocariers for Parkinson's disease treatment. |