Life Sciences & Biotechnology
Title : | Predicting the blood brain barrier permeability of gut microbes transformed low molecular weight marine poly(phenols) as attenuators of neuroinflammation via modulation of NF-κB signalling pathway. |
Area of research : | Life Sciences & Biotechnology |
Principal Investigator : | Dr. Maushmi Shailesh Kumar, S K Somaiya College, Maharashtra |
Timeline Start Year : | 2022 |
Timeline End Year : | 2025 |
Contact info : | maushmiskumar@gmail.com |
Equipments : | EPITHELIAL VOLTOHMMETER
EPITHELIAL VOLTOHMMETER |
Details
Executive Summary : | The gut microbiome and beta-amyloid peptide in the human body can significantly impact brain pathology, which is consistent with the neuropathogenesis of Alzheimer's disease (AD). Dietary poly(phenols) in the form of phlorotannins from brown macroalgae undergo biotransformation by gut microbiota to obtain metabolites active in the brain, which can directly act as neurotransmitters crossing the blood-brain barrier (BBB). These strong antioxidant compounds can enhance neuroprotection against brain inflammation. Two marine phlorotannins (dieckol and 8,8′-bieckol) from Ecklonia cava macroalgae effectively penetrate the brain through the BBB with low oral bioavailability. In the human body, they are well absorbed in the intestine and persist in the plasma for a substantial time, making them an effective prevention and treatment approach for neurodegenerative diseases.
Low-molecular weight (LMW) polyphenols have been found to attenuate neuroinflammation in stimulated microglia cells, chelate metals, scavenge free radicals, and inhibit acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) for the management of Alzheimer's disease. This study aims to investigate the nutritional phlorotannins as viable neuroprotective agents against inflammation, cholinergic enzymes, and metal chelation potential in AD. Sargassum wightii, an edible macroalgae with good antioxidant activity, will be examined for their inhibitory effect on amyloid beta 25-35 induced inflammatory response in human neuroblastoma SH-SY5Y cells. The study will stress the consumption of edible seaweed with minimal processing to prevent and reduce the occurrence of neurodegenerative disorders and contribute to the sustainable economy of seaweed cultivation in India. |
Total Budget (INR): | 29,89,096 |
Organizations involved