Executive Summary : | Climate change and pollution are critical factors in destroying ecosystems, including aquatic ones. These changes lead to declining production of aquatic organisms and contaminated final products. Researchers face challenges in alleviating these impacts on aquatic organisms, with mass mortality of fishes and contaminated harvested products observed. The global average land and ocean surface temperature has increased from 0.85°C to 0.78°C over the period 1850-1900 to 2003-2012, indicating that aquatic water bodies are getting hotter. Heavy metal toxicity increases at hotter water conditions, leading to water quality degradation and ammonia elevation. Toxicants like heavy metals in water pose serious risks to aquatic organisms and public safety. Contaminated fisheries and agricultural products can lead to diseases like cancer, blood pressure, and diabetes. The green revolution has been transformed into a greed revolution, but managing these changes in ecosystems requires understanding gene regulation involved in these conditions. Gene expression during multiple stress conditions can reveal ideas for mitigating or adopting species in such lethal conditions. This proposal aims to identify stress tolerance genes involved in multi-stress conditions in fish. Traditional trace metals, such as traditional trace metals, can be replaced with new effective metals like selenium and copper and their nanoparticles, which can significantly improve growth performance and contaminate-free harvested products. Monitoring growth performance can be accurately measured by gene expression of fish growth performance and immune-related genes. |