Executive Summary : | Microgels are known to perform as mimicking of extracellular matrix with flexible, dynamic and stimuli controlled soft materials. Herein, the proposal focuses on the fabrication of microgels in semi synthetic approach using chitosan as key building blocks, naturally occurring resource and biocompatible. The functionalities and interactions can be controlled by implantation of Hetero aromatic moieties (Quinazolines) using simple the covalent grafting on Chitosan. The on chitosan based microgels have a good combination of -NH2/-HC=N-, -OH (polyhydroxy) and hetero aromatic functionalities. The structural 3D gel-networks provide a highly porous nature, the diffusion ability makes them flowable in bloodstream, and dynamic functionalities develop self-healing behaviour. Gelations causes from H-bonding and also from the interactions of the other part depending on the incorporation of the functional group during preparation of sugar derivatives. FTIR, NMR (1H, 13C, Variable temperature dependent NMR), DLS, XRD diffraction and other various characterization techniques are used to establish the interesting features within Gel template. Gelation and the optical properties are depended on Interaction of solvent molecules and nature of hetero aromatic moieties. Furthermore, the proposed hydrogels will have appropriate water content and are deformable that can adapt the shape of the surface for the immobilization during application as bio-adhesive. The grafting of hetero aromatic moieties (pyrimidine, pyrazine, quinalozoline derivatives) on sugar backbone to form hydrogel assumed to be antibacterial potency. Popular and very useful DFT (density functional theory) estimation provides evidences to understand the electronic-environment, chemical-responses and scope of binding, chelation and encapsulation either within molecules or guest molecules. The optimized structures can be obtained to analyse the bonding -eatures during the interactions with protein etc. using docking approach. In a nut shell, the current proposal involves (i) preparation of a few microgels (ii) physicochemical-characterization (iii) bio-evaluation and (iv) detail computational analysis. Detail given in other technical details of the proposal. |