Life Sciences & Biotechnology
Title : | Investigating the role of andrographolide enhancing Micrococcus luteus OK271479 on the endomicrobiome and metabolic profile of Andrographis paniculata |
Area of research : | Life Sciences & Biotechnology |
Principal Investigator : | "Dr. Akanksha Singh, CSIR - Central Institute of Medicinal and Aromatic Plants (CIMAP), Lucknow, Uttar Pradesh " |
Timeline Start Year : | 2022 |
Timeline End Year : | 2025 |
Contact info : | akanksha@cimap.res.in |
Details
Executive Summary : | Andrographis paniculata (Burm.f.) Nees, known as the ‘king of bitters’ is reported to produce around 20 structurally similar diterpenoids and almost 10 flavonoids of pharmaceutical importance. Because of the presence of diverse group of important bioactive molecules the plant has been used to cure a range of diseases like fever, insect bite, hepatitis, common cold, inflammation ulcer etc. Out of all the reported bioactive molecules, andrographolide, a labdane diterpenoid is the most copious bioactive metabolite of the plant which has enormous therapeutic properties amongst which recently it has been found to be a potential inhibitor of SARS-CoV-2 main protease. However, the low yield of bioactive principles in kalmegh has led to augmented market rates for both raw materials which are being sold upto US$5/kg and purified compounds and their derivatives at the rate of US$100,000/kg. Thus, with the aim of enhancing the principal bioactive compound, andrographolide we plan to work on endophyte plant interaction as plants are associated with complex microbial communities which have an impact on host adaptation, plant fitness as well as primary and secondary metabolite contents. Among the various beneficial group of microorganisms associated with the plants; host associated endophytic microbiome represent an uncommon microbial community which is reported to change with time, genotype, biotic and abiotic elicitors. Since, genetic adaptation is comparatively slow in plants, the adjustment made by plant microbiome helps them to better equip under changing environmental conditions. In our recent study an endophyte Micrococcus luteus OK271479 isolated from A. paniculata seeds was found to significantly enhance the plant biomass, germination percentage as well as andrographanolide content (Unpublished). Since little is known about the mechanism; understanding the microbial partnership with A. paniculata at microbiome and metabolome level will improve our understanding of how plants for their own benefit modulate their native endomicrobiome in relationship with the chemistry of the plant. In addition, the leads obtained will help in developing a “customized bioformulation” for secondary metabolite enhancement in A. paniculata. |
Co-PI: | Dr. Ratnasekhar CH, CSIR - Central Institute of Medicinal and Aromatic Plants (CIMAP), Lucknow, Uttar Pradesh-226015 |
Total Budget (INR): | 59,03,539 |
Organizations involved