Life Sciences & Biotechnology
Title : | Development of RNA aptamer conjugated nanoparticle-5hmdC and 5fdC targeting cytidine deaminase expressing cancer cells |
Area of research : | Life Sciences & Biotechnology |
Focus area : | Enzymology |
Principal Investigator : | Dr. Gaurav Parashar, National Institute of Pharmaceutical Education and Research (NIPER), Mohali |
Contact info : | tikoo@niper.ac.in |
Details
Executive Summary : | Cloning, expression and purification of cytidine deaminase (CDA). CDA specific internalising RNA aptamer isolation using systemic evolution of ligands by exponential enrichment (SELEX), functional validation of enriched CDA specific aptamer in differentially expressing cell lines, synthesis of PLGA-PEG-COOH nanoparticle-aptamer conjugate containing modified nucleotides (5hmdC and 5fdC), and invitro and invivo evaluation of nanoparticle-aptamer conjugate for targeted delivery of 5hmdC and 5fdC and downstream effect on cancer proliferation |
Co-PI: | Dr. K B Tikoo, Professor, National Institute of Pharmaceutical Education and Research (NIPER), Mohali |
Total Budget (INR): | 18,70,000 |
Organizations involved
