Life Sciences & Biotechnology
Title : | Development of quantum antenna as molecular detection tool aid for Spatiotemporally controlled in-vivo oxygen sensing: A metaphase analysis |
Area of research : | Life Sciences & Biotechnology |
Principal Investigator : | Dr. Dinesh Veeran Ponnuvelu, Dhanalakshmi Srinivasan University, Tamil Nadu |
Timeline Start Year : | 2022 |
Timeline End Year : | 2024 |
Contact info : | vp.dinesh@gmail.com |
Equipments : | Mass flow controller |
Details
Executive Summary : | Using chemiluminescent mode of imaging biological systems especially cells and tissues provide an enormous information related to the biolabeling or biological tracking applications. Upconverting nanoparticles provide an excellent photon upconversion property from near-infrared (NIR) excitations into UV and visible emissions for not only with neural imaging, to NIR-light-controlled biosensing, activation of therapeutics and tumor-targeting. Also benefited from the high-sensitivity and capacity of real-time monitoring, luminescence imaging has been adopted as an excellent approach to detect the morphological, anatomical, and physiological details in cells and tissues. More importantly, through the control of the timing and location of the light irradiation, spatiotemporal control of the UCNPs sensor will be achieved for live imaging. To make it more bio-compatible, surface functionalization of UCNPs with dopamine nanoparticles, via passive or active targeting is carried out. Certain aspects of these NPs to be considered as imaging contrast agents are toxicity, stealth properties, specificity, range of excitability and emission and at last brightness. UCNPs would fill the gaps and be the best choice for live cell imaging and its simultaneous molecular detection of gases using porphyrin complexes. The exploitation of porphyrins as photosensitizers to enhance photocurrent intensity is related to their photoelectronic properties, like their wide photo-response range in visible and near-infrared regions and their quick time of the charge of recombination between the HOMO orbital and the hole of oxidized porphyrin. Herein, we report for the rational design of conjugating UCNPs with dopamine functionalized porphyrin complexes towards in-vitro gaseous detection with high resolution both temporally and spatially way and known as opto-electronic nose. This approach will open-up a new paradigm in optoelectronic sensors for biomedical usage with an aim to check the O2/CO2 levels on cell-head space for the actual in-situ monitoring of gas potential. Further as an extension, the sensing technology platform using functionalized UCNPs integrated with calorimetric platform insinuates the scenario for sensing direct imaging via optical sensor device. This investigation will contribute greatly towards the choice of the optoelectronic materials and process conditions of bio-compatible dopamine, device fabrication, and real time testing of the device in-Vitro. |
Total Budget (INR): | 27,64,340 |
Organizations involved