Life Sciences & Biotechnology
Title : | Development of novel high-level secretary expression systems and continuous production of anti-diabetic sugar, D-allulose |
Area of research : | Life Sciences & Biotechnology |
Focus area : | Exponential Technologies |
Principal Investigator : | Prof. Sudhir P. Singh, Gujarat Biotechnology University, Gujarat |
Timeline Start Year : | 2022 |
Timeline End Year : | 2025 |
Contact info : | pratapsudhir17@gmail.com |
Equipments : | Fermenter 3 L
Fermenter 3 L |
Details
Executive Summary : | D-allulose, a C-3 epimer of D-fructose, is a bio-safe and palatable sweetener with anti-diabetic, anti-obesity, anti-dyslipidemic, and neuroprotective potential. Its daily intake is comparable to that of caloric sugars like sucrose. D-allulose also improves the texture properties and shelf-life of food products. Chemical synthesis of D-allulose is cumbersome and generates undesirable chemical waste. To address this, biological systems for D-allulose production are needed. Ketose 3-epimerases, such as D-allulose 3-epimerase and D-tagatose 3-epimerase, are bacterial enzymes that catalyze the epimerization of D-fructose to D-allulose. The group demonstrated thermal stability improvement in a D-allulose 3-epimerase by following N-terminal fusion of Smt3 and enzyme immobilization approaches. They also identified a novel D-allulose 3-epimerae gene (daeM) from an extreme temperature thermal spring metagenome. Recently, a novel D-allulose 3-epimerase gene, daeB, was discovered in Bacillus subtilis, exhibiting profuse heat stability at 50°C and a high turnover number. However, translation of this invention is limited by low-level protein expression in heterologous hosts. The proposal aims to construct high-level expression systems with extracellular secretion of enzymes, establish a fermentation approach for enzyme production, immobilize the enzyme to a natural polymer matrix for continuous production of D-allulose, and develop a chemical method for precipitation. |
Co-PI: | Dr. Bhuwan Bhushan Mishra, Center Of Innovative And Applied Bioprocessing (CIAB), Mohali, Punjab-140306 |
Total Budget (INR): | 49,43,280 |
Patents : | 1 |
Organizations involved