Research
Title : | Development and application of double round multiplex polymerase chain reaction in the identification of microbial causes for prompt etiologic diagnosis of intra-vitreal aspirates from patients clinically diagnosed as endophthalmitis |
Area of Research : | Medical Sciences |
Focus Area : | Microbiology |
Principal Investigator : | Dr. R. Ramakrishnan, Professor, Aravind Eye Hospital and Postgraduate Institute of Ophthalmology, Tamil Nadu |
Co-PI: | Dr. S. Ramesh, Associate Professor and Head, Postgraduate Sri Paramakalyani College, Tamil Nadu, Dr. C. Shivkumar, Chief, Aravind Eye Hospital and Postgraduate Institute of Ophthalmology, Tamil Nadu, Dr. V. Maneksha, Chief, Aravind Eye Hospital and Postgraduate Institute of Ophthalmology, Tamil Nadu, Dr. Y. C. Venugopal Reddy, Chief, Aravind Eye Hospital and Postgraduate Institute of Ophthalmology, Tamil Nadu |
Contact info : | tvl.office@tvl.aravind.org |
Timeline Start Year : | 2012 |
Timeline End Year : | 2015 |
Details
Executive Summary : | To determine the utility of various polymerase chain reaction (PCR) methods in intra-ocular fluids for detecting the infectious genomes in the diagnosis of infectious endophthalmitis in terms of sensitivity and specificity. This prospective and consecutive analysis included a total of 326 intra-ocular fluids and four other ocular specimens that were submitted for the microbiological evaluation, which were obtained from 330 clinically diagnosed endophthalmitis patients presented between October 2012 and September 2015 at the tertiary eye care referral centre in South India. Part of the collected intra-ocular fluids were subjected to cultures and smears, and the remaining parts were utilized for five PCR uniplex, nested, semi-nested, multiplex and nested multiplex after extracting DNA, using universal eubacterial and Propionibacterium acnes speciesspecific primer sets targeting 16S rRNA gene in all bacteria and pan-fungal primers, targeting 28S rRNA gene in all fungi. |