Executive Summary : | The study aims to explore the scientific basis for using target plants in folklure medicine, specifically Artemisia moorcroftiana, Stachys tibetica, and Senecio graciliflorus. Artemisia moorcroftiana is used for malaria treatment, while Stachys tibetica is used for mental disorders. The three target plants are unexplored, and their phytochemistry and pharmacology will be evaluated using standard procedures and assays. Chemotaxonomic markers will be estimated using HPLC/LCMS. The most active isolated constituents will undergo structural modification for SAR studies to improve their activity and toxicity. The study will screen for cytotoxicity, antimalarial activity, and neuroprotection using appropriate assays like SRB, MMT, and cell survival assay against corticosterone-induced impairment in differentiated SH-SY5Y cells. Stachys tibetica is used for mental disorders and phobias, but no phytochemical studies have been reported. The study proposes phytochemical evaluation of Stachys tibetica and its constituents for neuroprotection against corticosterone-induced impairment in differentiated SH-SY5Y cells. Senecio graciliflorus, another target plant, has neuroprotective activity against corticosterone-induced impairment in differentiated SH-SY5Y cells. The researchers aim to fine-tune the most potent constituent through structural modification to develop a better neuroprotective analog. In conclusion, the study aims to unravel folklure claims associated with target plants at the molecular level and develop structural modifications for desired pharmacological effects. |