Life Sciences & Biotechnology

Title :

 Understanding the mechanism of degradation of cytoplasmic DNA containing alkyl-adducts

Area of research :

Life Sciences & Biotechnology

Focus area :

Cell biology, molecular biology

Principal Investigator :

Dr. Anindya Roy, Professor, Indian Institute of Technology(IIT), Hydrabad

Timeline Start Year :


Timeline End Year :


Contact info :


Executive Summary :

The study aims to investigate the molecular mechanism of cytoplasmic DNA degradation by TREX1, a nuclease involved in normal cell DNA regulation. The research suggests that cytosolic DNA might be endogenously alkylated and resist TREX1-mediated degradation, leading to the accumulation of alkylated DNA in the cytosol. The project will use high-resolution mass-spectrometry to examine endogenous alkylation in cytoplasmic DNA, synthetic oligonucleotides containing specific DNA alkyl-adducts, and in vitro assays to determine the effect of DNA containing abasic sites or methyl-adducts on CGAS activity.


Dr. Sandipan Ray, Indian Institute of Technology(IIT), Hydrabad

Total Budget (INR):


Organizations involved