Patents
This invention relates to a method for preparation of mycolytic enzymes containing mainly chitinase. The said process uses a fungus Myrothecium verrucaria. This mycolytic enzyme complex is made up of mainly cell wall hydrolytic enzymes, such as chitinases, glucanases and proteinases in significant quantities and in different proportion. The process steps are: growing Myrothecium sp. characterized in that on bean pod till it reaches to speculation stage, growing in a conventional fermentation medium having carbon and nitrogen sources and minor ingredients such as herein described for a period of at least 7 days in a known manner, separating the mycelia by conventional methods to obtain a mixture of mycolytic enzymes in the separated liquid, separating the product by known methods.