Agricultural residues (biomasses) have come-up as potential valuable renewable resources for transformation into many bio-products. To achieve this goal, the isolation of major biopolymers in their purest form through an economical process is demanded for synthesis of the target fine chemicals without impurities. However, the difficulty of fractionating the biomass into cellulose, hemicellulose and lignin by a simple method has greatly limited their isolation from agricultural residues. Also, the lipids and waxes present on the surface of the agricultural residues protect it from external chemical and microbial agents. To overcome this issue, the lipids and waxes have been isolated from the biomass through two step organic solvents extraction. Thereafter lignin, hemicellulose and cellulose are isolated successively from the de-waxed biomass.