Life Sciences & Biotechnology
Title : | Mechanistic analysis of tRNA-derived RNA fragments in DENV pathogenesis |
Area of research : | Life Sciences & Biotechnology |
Principal Investigator : | Dr. Bhupendra Kumar Verma, All India Institute Of Medical Sciences (AIIMS), New Delhi |
Timeline Start Year : | 2023 |
Timeline End Year : | 2026 |
Contact info : | bverma@gmail.com |
Details
Executive Summary : | Here we are focusing to characterize the role of novel small non-coding RNAs (sncRNAs) called tRNA half’s and tRNA derived RNA fragments in the pathogenesis of Dengue virus. Currently there is no treatment for DENV infection and there is immediate need to explore new therapeutic avenues. Dengue Virus (DENV), a member of flavivirus is the leading cause of arthropod-borne viral disease in world. The genome is single-stranded positive sense RNA. It can cause fever, DSS or hemorrhagic fever which can lead to visceral and central nervous system disease in human beings. There is no treatment of DENV infection yet and only tertiary care is available. There is immediate requirement to understand the molecular basis of disease. tRNA derived small RNA fragment (tsRNA) is the emerging class of small non coding RNAs which are produced as a result of action of different RNase such as Angiogenin, Dicer and ELAC2. These fragments are mainly generated under different stressed conditions. There are many regulatory roles associated with these tRNA derived small RNA fragments. These tRNA derived fragments may serve as useful biomarkers for DENV pathogenesis. We aim to characterize the mechanistic role of tRNA half’s in DENV disease manifestation. Our earlier study indeed suggests that the levels of RNases get modulated during DENV infection. Our results suggested that RNases knockdown cannot be achieved during DENV. We also observed that with the increase in RNases levels, viral replication also gets increased in parallel Our results hint the widespread and yet to be explored role of tRNA fragments. We will be investigating the generation of these tRNAs fragments during dengue pathogenesis and will further characterize their mechanism during DENV infection. Our study will reveal potential therapeutic roles of these tRNA derived RNA fragments and their possible use as biomarkers for severity of the disease. |
Total Budget (INR): | 46,78,696 |
Organizations involved