Life Sciences & Biotechnology
Title : | Understanding the mechanism of degradation of cytoplasmic DNA containing alkyl-adducts |
Area of research : | Life Sciences & Biotechnology |
Focus area : | Cell biology, molecular biology |
Principal Investigator : | Dr. Anindya Roy,
Professor, Indian Institute of Technology(IIT), Hydrabad |
Timeline Start Year : | 2023 |
Timeline End Year : | 2025 |
Contact info : | anindya@bt.iith.ac.in |
Details
Executive Summary : | The study aims to investigate the molecular mechanism of cytoplasmic DNA degradation by TREX1, a nuclease involved in normal cell DNA regulation. The research suggests that cytosolic DNA might be endogenously alkylated and resist TREX1-mediated degradation, leading to the accumulation of alkylated DNA in the cytosol. The project will use high-resolution mass-spectrometry to examine endogenous alkylation in cytoplasmic DNA, synthetic oligonucleotides containing specific DNA alkyl-adducts, and in vitro assays to determine the effect of DNA containing abasic sites or methyl-adducts on CGAS activity. |
Co-PI: | Dr. Sandipan Ray, Indian Institute of Technology(IIT), Hydrabad |
Total Budget (INR): | 41,82,000 |
Organizations involved
