Executive Summary : | Adenine (A), cytosine (C), guanine (G), thymine (T) and uracil (U) are the building blocks of RNA and DNA. They play a significant role in the storage of genetic information and protein biosynthesis. These nucleobases are liable for the DNA's stability, replication, and storage of information. Any kind of abnormal changes in these nucleobases can leads to various diseases including epilepsy, cancer, tumorigenesis, mental retardation, HIV infection, Parkinson's disease, carcinoma and liver diseases. Therefore, to diagnose various diseases in their early stage qualitative and quantitative analysis of nucleobases concentration is considered crucial, as they can reflect the mutation and level of oxidative damage to DNA. For that purpose, a novel probe for detection of individual nucleobase, with easy fabrication as well as high response and good selectivity, is urgently desirable both in academic research and in clinical diagnosis. Rapid and low-cost detection of nucleobases is significant for practical applications, and fluorometric sensing offers all these advantages above the other analytical techniques. Also, fluorescence based bio-sensing has been showing promise in the diagnosis and prognosis of diseases, due to their fast response, easy handling, and detection of even trace analyte. In this perspective, metal organic framework (MOFs) i.e inorganic-organic hybrid materials have gain a lot of attention and signifying unique advantages along with high capabilities, in biosensing applications. As they offer made-to-order structures, permanent porosity, high surface area and superb biocompatibility. These hybrid materials have been explored for many applications in different areas such as catalysis, medicine, sorption, and magnetism etc. MOFs as fluorometric probes have shown unlimited potential for various analytes detection. These probes possess superior guest recognition capabilities along with specific optical response for subsequent analyte. Hence selectivity and sensitivity can be achieved. Depending on the nature of electronic environment of the analyte and/or MOFs, it shows two types of behaviour i.e : a) “turn-off sensing and b) ”turn-on“ sensing, which can be detected fluorometrically. At all, MOF based sensor offers easy, visible and cost effective sensing. So far no reports on MOF based fluorometric sensing of individually all nucleobases, have been reported. In this project we propose new MOFs for detection of DNA nucleobases. The current proposal gives an opportunity for real-time and low-cost detection of nucleobases using MOF fluorometric probe. |