Research
Title : | FRET/FLIM-FRET technique to investigate the role of hydrophobic and hydrophilic gold nanoclusters in modulating vesicle fusion and determination of the thickness of lipid bilayer |
Area of research : | Chemical Sciences |
Principal Investigator : | Prof. Pradipta Purkayastha, Indian Institute Of Science Education And Research (IISER) Kolkata, West Bengal |
Timeline Start Year : | 2022 |
Timeline End Year : | 2025 |
Contact info : | ppurkayastha@iiserkol.ac.in |
Equipments : | Time-resolved spectrometer with picoseconds resolution |
Details
Executive Summary : | The study aims to synthesize ultrasmall, fluorescent glutathione-protected gold nanoclusters (Au NCs) that can be placed on the surface and deep inside lipid bilayers. The Au NCs absorb light at 405 nm and emit at 600 nm. Methylene blue (MB) will be incorporated into the lipid vesicles during the synthesis, producing a good FRET pair. Differential FRET with MB will occur depending on the Au NCs' position. The FRET parameters will provide bilayer thickness distances. The study will use steady state and time-resolved fluorescence measurements and fluorescence lifetime imaging microscopy for detailed dynamics. |
Total Budget (INR): | 67,13,696 |
Organizations involved
Implementing Agency : | Indian Institute Of Science Education And Research (IISER) Kolkata, West Bengal Funding Agency :Anusandhan National Rsearch Foundation (ANRF)/Science and Engineering Research Board (SERB) | Source :Science and Engineering Research Board (SERB), DST 2022-23 | |