Life Sciences & Biotechnology
Title : | Early Diagnosis of Infection Biomarkers (human neutrophil elastase (HNE) and cathepsin G (CatG)) in Wound Fluid based on Textile-based Embroidered Biosensors |
Area of research : | Life Sciences & Biotechnology |
Principal Investigator : | Dr. Tamal Sarkar, Indian Institute Of Technology (IIT) Kharagpur, West Bengal |
Timeline Start Year : | 2022 |
Timeline End Year : | 2024 |
Contact info : | tamalsarkar09@gmail.com |
Details
Executive Summary : | Wound infection is a significant issue during the healing process, causing delays in healing, skin flushing, swelling, pain, and impaired function. To address this, researchers propose detecting enzymes produced by pathogenic organisms at a stage where conventional methods are time-consuming and uncertain. Neutrophil enzymes, such as human neutrophil elastase (HNE) and cathepsin G (CatG), are produced at the early stage of infection, and their analysis determines the wound's condition. Peptide substrates for HNE and CatG are immobilized on different surfaces to monitor their activity in the wound fluid medium. The researchers propose using carbon conducting yarn (CCY) as wearable enzyme sensors to detect these enzymes. CCY is an excellent class of carbon material with carbon atoms bonded together in crystals arranged parallel to the fiber's long axis. The CCY will be functionalized using graphene quantum dots (GQDs) and L-Cystine capped Fe₃O₄ NPs, allowing biomolecules (antibodies) to be immobilized for biosensing. The proposed project focuses on developing textile-based embroidered sensors for simultaneous electrochemical detection of HNE and CatG enzyme biomarkers for wound infection. The sensors will be integrated onto a fabric attachable to the wound point through an embroidery technique, and a prototype device with wireless monitoring ability will be fabricated for wound infection diagnosis. |
Organizations involved