Research

Medical Sciences

Title :	Development of SERS based ultra-sensitive, rapid, portable and field deployable platform for detection of pathogenic bacteria and Dengue virus
Area of Research :	Medical Sciences, Life Sciences & Biotechnology
Focus Area :	Maternal and child health, Point of care diagnostic tests and surveillance systems for common non-communicable and Infectious diseases
Principal Investigator :	Dr. J.P. Singh, Professor, Department of Physics, Indian Institute of Technology (IIT), New Delhi
Co-PI:	Prof. Prashant Mishra, Professor, Biochemical Engineering and Biotechnology, Indian Institute of Technology (IIT), New Delhi, Dr. Neena Valecha, Director & Scientist-G, National Institute of Malaria Research (NIMR), New Delhi, Dr. Deepali Savargaonkar, Scientist-D, National Institute of Malaria Research (NIMR), New Delhi
Contact info :	jpsingh[at]physics[dot]iitd[dot]ac[dot]in; pmishra[at]dbeb[dot]iitd[dot]ac[dot]in; director[at]mrcindia[dot]org;
Total Budget (INR):	1,22,16,000

Details

Executive Summary :

A new handheld, field-deployable SERS based diagnostic tool having capabilities of rapid on-chip specimen separation and detection of Dengue virus and pathogenic bacteria like Staphylococcus aureus and Escherichia coli for point-of-care diagnostics will be developed. The current available methods for identification of bacteria/virus such as biochemical test, PCR are time consuming and laborious. The novel Ag nanorod based surface enhanced Raman scattering substrates fabricated by e-beam glancing angle deposition (GLAD) system will provide rapid and accurate detection of pathogenic bacteria and dengue virus. The hypothesis is that SERS spectrum provides a unique 'molecular fingerprint' of individual virus/bacteria based on their specific molecules that are in contact with metal nanoparticles without any species-specific reagents typically required for virus/bacteria signal amplification.

Organizations involved

Implementing Agency :	Indian Institute of Technology (IIT), New Delhi National Institute of Malaria Research (NIMR), New Delhi
Funding Agency :	Ministry of Health and Family Welfare (MoHFW), Govt. of India