Life Sciences & Biotechnology
Title : | Development of nanomaterial decorated gelatin-polymannose hydrogel based three-dimensional culture for drug testing |
Area of research : | Life Sciences & Biotechnology |
Principal Investigator : | Dr. Arul Prakash F, Saveetha Dental College And Hospital, Tamil Nadu |
Timeline Start Year : | 2022 |
Timeline End Year : | 2024 |
Contact info : | fdapharma@gmail.com |
Equipments : | CO2 Incubators
Rotary Vane Vacuum Pump |
Details
Executive Summary : | Cancer incidence and mortality are rapidly increasing worldwide, with breast and lung cancer being the most diagnosed types. Despite advancements in scientific knowledge and increased therapeutic choices, patient survival rates have not improved. New treatments are needed for this deadly disease, and an effective platform for screening multiple drugs is needed. Current strategies used to screen anti-cancer drugs include clinical samples (biopsies) and animal models in vitro and in vivo. However, these models have drawbacks like low-throughput, cost-intensive, and difficult to scale up. To overcome these challenges, 3D cultures models can be used to mimic the in vivo environment. These three-dimensional (3D) models allow the investigation of more variables that affect tumor growth, invasion, and metastasis. High throughput drug screening that is not possible using in vivo animal models can be achieved with 3D cultures. The present study proposes creating novel, cost-effective 3D culture models using graphene decorated gelatin-polymannose (NGPM) based hydrogels to screen anti-cancer drugs. The objectives of this study include providing a versatile hydrogel 3D culture system for drug testing application, preparing and characterizing NGPM hydrogel, developing A549 3D cultures using NGPM hydrogels, investigating the integrity of the culture using RT-PCR/Western blots, and determining the efficiency of Engineered NGPM-Hydrogel 3D cultures towards drug testing. The long-term goal is to develop and market cheaper and effective NGPM- hydrogel 3D cultures for screening drugs for various diseases. |
Total Budget (INR): | 28,12,287 |
Organizations involved