Life Sciences & Biotechnology
Title : | Investigating the role of hypoxia in regulating differentiation and development of leukemia |
Area of research : | Life Sciences & Biotechnology |
Principal Investigator : | Prof. Tapasya Srivastava, University Of Delhi, South Campus, Delhi |
Timeline Start Year : | 2022 |
Timeline End Year : | 2025 |
Contact info : | tapasya@south.du.ac.in |
Equipments : | Machine for giving regulated hypoxia |
Details
Executive Summary : | Preeclampsia (PE) is a pregnancy-specific disorder causing complications in 7-10% of pregnancies and leading to maternal and perinatal morbidity and mortality. It is classified as early-onset or late-onset preeclampsia, depending on the time. The etiology and pathogenesis of PE are not fully understood. Studies have shown dysregulation of the complement system in recurrent pregnancy loss, preterm birth, preeclampsia, hypertensive disorders of pregnancy, and intrauterine growth restriction. Factor H (FH), a key complement inhibitor, affects the C3bBb convertase and regulates C3b-containing C5 convertases. This investigation aims to understand the role of FH in placental dysfunction in cases of early onset preeclampsia (EOPE), late onset preeclampsia (LOPE), and normal pregnancy (NP). The expression and localization of FH in placental tissue will be assessed using immunohistochemistry and RT-PCR techniques. The study will also investigate FH's role in immuodulation, using cultured ACD explants exposed to IL-1β/LPS with and without Factor H. Syncytiotrophoblast derived extracellular vesicles (STBEVs) have been found to exhibit immunomodulatory roles during pregnancy and increase in PE compared to normal pregnancy. The study aims to determine whether FH is associated with placental STBEVs and whether it is differentially expressed in PE and NP samples. The study can help identify novel protein markers for diagnosis of PE and their roles in PE pathogenesis. |
Co-PI: | Prof. Sameer Bakhshi, All India Institute Of Medical Sciences (AIIMS), New Delhi-110029 |
Total Budget (INR): | 40,08,422 |
Organizations involved