Life Sciences & Biotechnology
Title : | Fabrication and Biological Assessment of Fucoidan-Garlic extract Coated Quercetin Nanoparticles as Synergistic Anti-Leishmanial Agents |
Area of research : | Life Sciences & Biotechnology |
Principal Investigator : | Dr. Piyush Kumar Gupta, Sharda University, Uttar Pradesh |
Timeline Start Year : | 2022 |
Timeline End Year : | 2024 |
Contact info : | dr.piyushkgupta@gmail.com |
Equipments : | Vacuum Pump
Single channel microliter pipettes
pH meter |
Details
Executive Summary : | Leishmaniasis is a zoonotic parasitic disease caused by Leishmania, transmitted through the bites of infected sandflies. The primary treatment for Leishmaniasis is chemotherapy, which is expensive and toxic, requiring lengthy hospitalization and burdening weak economies. Quercetin (QC), a penta-hydroxyl flavone, has numerous pharmacological effects, including antileishmanial, antibacterial, antioxidant, antiviral, and anticancer activity. Garlic extract and Fucoidan (FD) also possess diverse pharmacological activities like QC. However, their oral absorption is slow due to their hydrophobic nature. Therefore, there is a need to encapsulate these lipophilic bioactive molecules in lipid-based nanocarriers to enhance their oral solubility and stability, increasing their intestinal uptake and reducing the drug's dose due to the synergistic effect with excipients. The objectives of this study include designing a targeted drug delivery system to solve the problem of QC's solubility and bioavailability, determining the optimum concentration of QC and excipients for the preparation of fucoidan-garlic extract coated quercetin nanoparticles (FGQN), studying in silico pharmacokinetic studies, and investigating the antileishmanial activity of QC, GE, FD individually and the synergistic effect of optimized FGQ nanoparticles. The strategy will employ the amalgamation of FD and GE, along with specific lipids/fatty acids, transformed into nanoparticles for ferrying QC. This will not only avoid the unnecessary introduction of inactive excipients in the body but also reduce the drug's dose with enhanced solubility and bioavailability due to the synergistic effect of excipients and drugs. |
Total Budget (INR): | 23,78,164 |
Organizations involved