Executive Summary : | Wound healing is a complex process that requires support for angiogenesis, wound healing factors, and cell proliferation and migration. Mesenchymal stem cells (MSCs) are well-accepted for therapeutic applications in neurological and cardiac disorders due to their non-immunogenic nature. However, cell therapy has faced limitations, such as cell viability. MSCs secrete several immunomodulatory proteins, which contribute to their non-immunogenic nature. The cell secretome, which is interdependent and modulated in-vivo based on physiological signals, can be targeted for better therapeutic applications and remove limitations of cellular therapy. In recent years, attention has shifted to MSCs' secretome, which can be optimized by culture conditions, induced by cytokines, or combined with other cells. Platelets, small anucleated circulating blood cells, play a crucial role in haemostasis, inflammation, and wound healing. They release growth factors and signaling molecules, such as PDGF, VEGF, ADP, and cytokines, which modulate other cells to respond via signaling pathways. PRP releases at appropriate concentrations promote cell proliferation, migration, and angiogenesis in a better and controlled way. Platelets can enhance wound healing capacity by signaling and transferring mitochondrial RNA to alter metabolic activity, resulting in the secretion of angiogenic factors by MSCs. Cell secretome differs significantly based on culture conditions, with 3D cultures showing cellular secretome closer to in-vivo conditions. Cell cultured in spheroid environments showed better cell morphology and migration compared to 2D cultures. The proposed approach involves induced MSCs through circulating platelets in spheroid culture to enhance/modulate wound regeneration behaviors. |